Normal Fibroblast Cell Line

Normal human atcc pcs 201 013 from the liquid nitrogen tank.

Normal fibroblast cell line.

Panel i tumor protein tp73 and prdm16 megf6 cep104 and dffb expression level close to the tp73 gene located in chromosome 1p36 32 region ii related genes. Make sure that the caps of all components are tight thaw the components of fibroblast growth kit low serum atcc pcs 201 041 just prior to adding them to the fibroblast basal medium atcc pcs 201 030. Confluent fibroblast monolayer obtained on the 3 rd day of seeding figure figure1a 1a c c showed normal karyotype figure 2. These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82.

Initiated in september 1966 by j. Primary normal human dermal fibroblasts nhdf are isolated form the dermis of juvenile foreskin or adult skin from different locations like the face the breasts the abdomen and the thighs. But after several passage this fibroblasts transform to cells that have features of cancerous cells. The immunohistological pattern of fibroblast cell differentiation was established using specific markers such as vimentin s100a4 tgfβr1 and phalloidin.

This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol so called 3t3 protocol. Fibroblast cell line. The primary mouse embryonic fibroblast cells were transferred the t every 3 days the first 3 and inoculated at the rigid density of 3 10 5 cells per 20 cm 2 dish the. Complete growth medium obtain one vial of primary lung fibroblast cells.

They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma fibrosarcoma fibrosis xeroderma. This encoded protein is associated with a psoriasis like skin phenotype. This protein interacts with the egf tgf alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. B expression of tp73 gene and related genes in normal human liver stem cells hepcy hepco hcc gi cancer cell lines and normal human skin fibroblasts cell line hs27.

The bj cell line has a long lifespan in comparison with other normal human fibroblast cell lines. Jacobs the cell line was derived from the human lung tissue of a 14 week old male fetus aborted from a 27 year old woman. L929 is a normal fibroblast cell line from subcutaneous connective tissue of mouse. Provided by refseq jul 2008.

A frozen ampule at population doubling 2 3 was received at the atcc in april 2000.